Biomechanical changes in the sclera of monkey eyes exposed to chronic IOP elevations

Invest Ophthalmol Vis Sci. 2011 Jul 29;52(8):5656-69. doi: 10.1167/iovs.10-6927.


Purpose: To characterize scleral biomechanics in both eyes of eight monkeys in which chronic intraocular pressure (IOP) elevation was induced in one eye.

Methods: Each posterior sclera was mounted on a pressurization apparatus, IOP was elevated from 5 to 45 mm Hg while the 3D displacements of the scleral surface were measured by speckle interferometry. Finite element (FE) models of each scleral shell were constructed that incorporated stretch-induced stiffening and multidirectionality of the collagen fibers. FE model predictions were then iteratively matched to experimental displacements to extract unique sets of scleral biomechanical properties.

Results: For all eyes, the posterior sclera exhibited inhomogeneous, anisotropic, nonlinear biomechanical behavior. Biomechanical changes caused by chronic IOP elevation were complex and specific to each subject. Specifically: (1) Glaucomatous eyes in which the contralateral normal eyes displayed large modulus or thickness were less prone to biomechanical changes; (2) glaucomatous scleral modulus associated with an IOP of 10 mm Hg decreased (when compared with that of the contralateral normal) after minimal chronic IOP elevation; (3) glaucomatous scleral modulus associated with IOPs of 30 and 45 mm Hg increased (when compared with that of the contralateral normal) after moderate IOP elevation; and (4) FE-based estimates of collagen fiber orientation demonstrated no change in the glaucomatous eyes.

Conclusions: Significant stiffening of the sclera follows exposure to moderate IOP elevations in most eyes. Scleral hypercompliance may precede stiffening or be a unique response to minimal chronic IOP elevation in some eyes. These biomechanical changes are likely to be the result of scleral extracellular matrix remodeling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Anisotropy
  • Biomechanical Phenomena / physiology
  • Chronic Disease
  • Compliance / physiology
  • Disease Models, Animal
  • Elasticity / physiology
  • Extracellular Matrix / physiology
  • Glaucoma / pathology
  • Glaucoma / physiopathology*
  • Intraocular Pressure / physiology*
  • Macaca mulatta
  • Models, Biological*
  • Nonlinear Dynamics
  • Sclera / pathology
  • Sclera / physiology*
  • Tonometry, Ocular