1H, 13C, and 15N NMR assignments of the Pyrococcus abyssi DNA polymerase II intein

Biomol NMR Assign. 2011 Oct;5(2):233-5. doi: 10.1007/s12104-011-9307-4. Epub 2011 Apr 26.


Protein splicing is a precise post-translational process mediated by inteins. Inteins are intervening proteins that cleave themselves from a precursor protein while joining the flanking sequences. Here we report the (15)N, (13)C, and (1)H chemical shift assignments of the intein from DNA polymerase II of Pyrococcus abyssi (Pab PolII intein), which has been recombinantly overexpressed and isotopically labeled. The NMR assignments of Pab PolII intein are essential for solution structure determination and protein dynamics study.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • DNA Polymerase II / chemistry*
  • Inteins*
  • Isotopes
  • Nuclear Magnetic Resonance, Biomolecular*
  • Pyrococcus abyssi / enzymology*
  • Recombinant Proteins / chemistry


  • Isotopes
  • Recombinant Proteins
  • DNA Polymerase II