Bifunctional dendronized cellulose surfaces as biosensors

Biomacromolecules. 2011 Jun 13;12(6):2114-25. doi: 10.1021/bm200201y. Epub 2011 May 9.


Well-defined dendronized cellulose substrates displaying multiple representations of dual-functionality were constructed by taking advantage of the efficiency of the click reaction combined with traditional anhydride chemistry. First, activated cellulose surfaces were decorated with several generations of dendrons, and their peripheral reactive groups were subsequently reacted with a trifunctional orthogonal monomer. The generated substrate tool box was successfully explored by accurately tuning the surface function using a versatile orthogonal dual postfunctionalization approach. In general, the reactions were monitored by using a click-dye reagent or a quartz crystal microbalance (QCM) technique, and the resulting surfaces were well-characterized using XPS, FT-IR, and contact angle measurements. Utilizing this approach two different surfaces have been obtained; that is, triethylenglycol oligomers and amoxicillin molecules were efficiently introduced to the dendritic surface. As a second example, mannose-decorated hydroxyl functional surfaces illustrated their potential as biosensors by multivalent detection of lectin protein at concentration as low as 5 nM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amoxicillin / chemistry
  • Amoxicillin / metabolism
  • Biomimetic Materials / chemical synthesis*
  • Biomimetic Materials / metabolism
  • Biosensing Techniques / methods*
  • Cellulose / analogs & derivatives
  • Cellulose / chemical synthesis*
  • Cellulose / metabolism
  • Click Chemistry
  • Dendrimers / chemical synthesis*
  • Dendrimers / metabolism
  • Lectins / analysis
  • Mannose / chemistry
  • Mannose / metabolism*
  • Photoelectron Spectroscopy
  • Polyethylene Glycols / chemistry
  • Polyethylene Glycols / metabolism
  • Quartz Crystal Microbalance Techniques
  • Spectroscopy, Fourier Transform Infrared
  • Surface Properties


  • Dendrimers
  • Lectins
  • triethylene glycol
  • Polyethylene Glycols
  • Amoxicillin
  • Cellulose
  • Mannose