Two-photon absorption properties of fluorescent proteins

Nat Methods. 2011 May;8(5):393-9. doi: 10.1038/nmeth.1596. Epub 2011 Apr 28.


Two-photon excitation of fluorescent proteins is an attractive approach for imaging living systems. Today researchers are eager to know which proteins are the brightest and what the best excitation wavelengths are. Here we review the two-photon absorption properties of a wide variety of fluorescent proteins, including new far-red variants, to produce a comprehensive guide to choosing the right fluorescent protein and excitation wavelength for two-photon applications.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Lasers
  • Luminescent Proteins / chemistry*
  • Luminescent Proteins / genetics
  • Microscopy, Confocal / methods
  • Photons*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Spectrophotometry / methods*
  • Static Electricity


  • Luminescent Proteins
  • Recombinant Proteins