Release of RNA polymerase from vero cell mitochondria after herpes simplex virus type 1 infection

J Virol. 1990 Jan;64(1):450-2. doi: 10.1128/JVI.64.1.450-452.1990.


Infection of Vero cells with herpes simplex virus type 1 results in the appearance in soluble extracts of a DNA primase activity. The partially purified enzyme, Mr, approximately 100,000, is identical in resistance to alpha-amanitin, pH profile, Mg2+ dependence, salt sensitivity, and KmATP to the catalytic core of Vero cell mitochondrial RNA polymerase. Moreover, the products synthesized are those expected of an RNA polymerase rather than a DNA primase. Inasmuch as the enzyme is not present in soluble extracts of uninfected Vero cells, we presume that the specific appearance of RNA polymerase in extracts of herpesvirus-infected cells results from infection-induced disruption of the mitochondrial membrane, followed by release of the enzyme into the cytosol.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Transformation, Viral*
  • DNA-Directed RNA Polymerases / biosynthesis*
  • DNA-Directed RNA Polymerases / isolation & purification
  • DNA-Directed RNA Polymerases / metabolism
  • Kinetics
  • Mitochondria / enzymology*
  • Simplexvirus / enzymology
  • Simplexvirus / genetics*
  • Vero Cells


  • DNA-Directed RNA Polymerases