Assembly of bacterial ribosomes

Annu Rev Biochem. 2011;80:501-26. doi: 10.1146/annurev-biochem-062608-160432.

Abstract

The assembly of ribosomes from a discrete set of components is a key aspect of the highly coordinated process of ribosome biogenesis. In this review, we present a brief history of the early work on ribosome assembly in Escherichia coli, including a description of in vivo and in vitro intermediates. The assembly process is believed to progress through an alternating series of RNA conformational changes and protein-binding events; we explore the effects of ribosomal proteins in driving these events. Ribosome assembly in vivo proceeds much faster than in vitro, and we outline the contributions of several of the assembly cofactors involved, including Era, RbfA, RimJ, RimM, RimP, and RsgA, which associate with the 30S subunit, and CsdA, DbpA, Der, and SrmB, which associate with the 50S subunit.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • DEAD-box RNA Helicases / genetics
  • DEAD-box RNA Helicases / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Escherichia coli Proteins / chemistry
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism*
  • GTP Phosphohydrolases / metabolism
  • Models, Molecular
  • Molecular Chaperones / genetics
  • Molecular Chaperones / metabolism
  • Nucleic Acid Conformation
  • Protein Conformation
  • Protein Subunits / chemistry
  • Protein Subunits / genetics
  • Protein Subunits / metabolism
  • RNA, Bacterial / chemistry
  • RNA, Bacterial / genetics
  • RNA, Bacterial / metabolism
  • RNA, Ribosomal / chemistry
  • RNA, Ribosomal / genetics
  • RNA, Ribosomal / metabolism
  • Ribosomal Proteins / chemistry
  • Ribosomal Proteins / genetics
  • Ribosomal Proteins / metabolism
  • Ribosomes / chemistry
  • Ribosomes / genetics
  • Ribosomes / metabolism*

Substances

  • Escherichia coli Proteins
  • Molecular Chaperones
  • Protein Subunits
  • RNA, Bacterial
  • RNA, Ribosomal
  • Ribosomal Proteins
  • GTP Phosphohydrolases
  • DEAD-box RNA Helicases