Ultra-performance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis

Anal Biochem. 2011 Aug 1;415(1):59-66. doi: 10.1016/j.ab.2011.04.003. Epub 2011 Apr 15.

Abstract

A high-resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultra-performance liquid chromatography in a reverse-phase ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues, and biological fluids, because it provides high sensitivity without being subject to interference from proteins, peptides, and other sample impurities.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Camelus
  • Chromatography, Liquid / methods*
  • Cricetinae
  • Cricetulus
  • Disaccharides / analysis*
  • Disaccharides / isolation & purification
  • Heparin / analogs & derivatives*
  • Heparin / analysis
  • Heparin / isolation & purification
  • Heparin Lyase / metabolism
  • Heparin, Low-Molecular-Weight / analysis
  • Heparitin Sulfate / analysis
  • Spectrometry, Mass, Electrospray Ionization / methods*

Substances

  • Disaccharides
  • Heparin, Low-Molecular-Weight
  • heparin disaccharide
  • Heparin
  • Heparitin Sulfate
  • Heparin Lyase