Extraction and normal-phase HPLC-fluorescence-electrospray MS characterization and quantification of procyanidins in cranberry extracts

J Food Sci. 2010 Oct;75(8):C690-6. doi: 10.1111/j.1750-3841.2010.01799.x. Epub 2010 Sep 24.


Cranberries are a major source of procyanidins, an abundant class of bioactive polyphenols found in nature. The objective of this study was to apply a protocol for extraction and normal-phase high-performance liquid chromatograph-fluorescence-mass spectroscopy separation and quantification of procyanidins in cranberry extracts containing varying processing aids and/or methods of production. Cranberry extracts were best extracted using an acetone/water technique versus an acid/alkaline extraction. Procyanidins were semi-purified with C-18 and Sephadex LH-20 SPE cartridges (GE Healthcare Life Sciences, Piscataway, N.J., U.S.A.). Characterization and quantification of procyanidins up to octamers and higher molecular weight compounds, including separation of the A- and B-type dimers to tetramers was achieved.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Chromatography, High Pressure Liquid
  • Fruit / chemistry*
  • Plant Extracts / chemistry*
  • Plant Extracts / isolation & purification*
  • Polymers / analysis
  • Polymers / chemistry
  • Proanthocyanidins / analysis*
  • Proanthocyanidins / chemistry
  • Solid Phase Extraction / methods
  • Spectrometry, Fluorescence
  • Spectrometry, Mass, Electrospray Ionization
  • Vaccinium macrocarpon / chemistry*


  • Plant Extracts
  • Polymers
  • Proanthocyanidins