Nucleotide sequence and transcriptional analysis of the Escherichia coli agp gene encoding periplasmic acid glucose-1-phosphatase

J Bacteriol. 1990 Feb;172(2):802-7. doi: 10.1128/jb.172.2.802-807.1990.


The nucleotide sequence of the agp gene, which encodes a periplasmic glucose-1-phosphatase, was determined. The deduced amino acid sequence corresponds to a 413-amino-acid-residue polypeptide with a typical hydrophobic signal sequence of 22 amino acids. The mature protein lacks the N-terminal signal peptide and has a calculated Mr of 43,514. Its promoter was defined by primer extension of the mRNA made in vivo. Like many genes under positive control, its -35 promoter region does not match the consensus. The agp gene is both preceded and followed by transcription termination signals, so it appears to be transcribed as a single unit.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Bacterial / genetics
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli Proteins*
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Phosphoric Monoester Hydrolases / genetics*
  • Plasmids
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • Restriction Mapping
  • Transcription, Genetic


  • DNA, Bacterial
  • Escherichia coli Proteins
  • RNA, Messenger
  • agp protein, E coli
  • Phosphoric Monoester Hydrolases

Associated data

  • GENBANK/M33807