Retinoic acid rapidly decreases phosphatidylinositol turnover during neuroblastoma cell differentiation

J Neurochem. 1990 Feb;54(2):540-6. doi: 10.1111/j.1471-4159.1990.tb01905.x.


Phosphatidylinositol (PI) turnover has recently been implicated in the regulation of cell proliferation and transformation. We have investigated its role in differentiation using LAN-1 cells, a human neuroblastoma cell line that can be induced to differentiate along the neuronal pathway by retinoic acid (RA). We have found that treatment of LAN-1 cells with RA is followed by a rapid decrease of inositol phospholipid metabolism, using myo-[1,2-3H]inositol or [1(3)-3H]glycerol. No changes were observed in both [3H]inositol and [3H]glycerol uptake within 24 h of RA treatment. Decreased incorporation of the metabolic precursor into PI 4-monophosphate and PI 4,5-bisphosphate occurred within 1 h of RA treatment. No changes were seen in the specific radioactivity of the precursor pools up to 1 h of treatment with RA. Analysis of labeled PI metabolites from prelabeled cells indicated a rapid decrease of inositol 1,4,5-trisphosphate and 1,2-diacylglycerol content within 1 min of induction of LAN-1 cell differentiation. These findings constitute the earliest reported events in neuroblastoma cell differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Deoxyglucose / metabolism
  • Glycerol / metabolism
  • Humans
  • Inositol / metabolism
  • Inositol Phosphates / metabolism
  • Neuroblastoma / metabolism*
  • Neuroblastoma / pathology
  • Phosphatidylinositols / metabolism*
  • Tretinoin / pharmacology*
  • Tumor Cells, Cultured


  • Inositol Phosphates
  • Phosphatidylinositols
  • Inositol
  • Tretinoin
  • Deoxyglucose
  • Glycerol