Abstract
We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Acids / chemistry*
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Carbohydrate Conformation
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Cations / chemical synthesis
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Cations / chemistry
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Cell Survival / drug effects
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Cells, Cultured
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Dextrans / chemical synthesis
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Dextrans / chemistry*
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Dextrans / pharmacology
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Dose-Response Relationship, Drug
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Drug Delivery Systems / methods*
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HeLa Cells
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Humans
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Hydrogen-Ion Concentration
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Particle Size
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RNA, Small Interfering / administration & dosage*
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RNA, Small Interfering / pharmacology
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Structure-Activity Relationship
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Surface Properties
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Transfection / methods*
Substances
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Acids
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Cations
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Dextrans
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RNA, Small Interfering