Adipose triglyceride lipase and hormone-sensitive lipase are involved in fat loss in JunB-deficient mice

Endocrinology. 2011 Jul;152(7):2678-89. doi: 10.1210/en.2010-1477. Epub 2011 May 3.


Proteins of the activator protein-1 family are known to have roles in many physiological processes such as proliferation, apoptosis, and inflammation. However, their role in fat metabolism has yet to be defined in more detail. Here we study the impact of JunB deficiency on the metabolic state of mice. JunB knockout (JunB-KO) mice show markedly decreased weight gain, reduced fat mass, and a low survival rate compared with control mice. If fed a high-fat diet, the weight gain of JunB-KO mice is comparable to control mice and the survival rate improves dramatically. Along with normal expression of adipogenic marker genes in white adipose tissue (WAT) of JunB-KO mice, this suggests that adipogenesis per se is not affected by JunB deficiency. This is supported by in vitro data, because neither JunB-silenced 3T3-L1 cells nor mouse embryonic fibroblasts from JunB-KO mice show a change in adipogenic potential. Interestingly, the key enzymes of lipolysis, adipose triglyceride lipase and hormone-sensitive lipase, were significantly increased in WAT of fasted JunB-KO mice. Concomitantly, the ratio of plasma free fatty acids per gram fat mass was increased, suggesting an elevated lipolytic rate under fasting conditions. Furthermore, up-regulation of TNFα and reduced expression of perilipin indicate that this pathway is also involved in increased lipolytic rate in these mice. Additionally, JunB-KO mice are more insulin sensitive than controls and show up-regulation of lipogenic genes in skeletal muscle, indicating a shuttling of energy substrates from WAT to skeletal muscle. In summary, this study provides valuable insights into the impact of JunB deficiency on the metabolic state of mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes, White / cytology
  • Adipocytes, White / metabolism*
  • Adiposity*
  • Animals
  • Carrier Proteins
  • Crosses, Genetic
  • Dietary Fats / administration & dosage
  • Gene Expression Regulation
  • Growth Disorders / diet therapy
  • Growth Disorders / genetics
  • Growth Disorders / metabolism*
  • Growth Disorders / mortality
  • Insulin Resistance
  • Lipase / genetics
  • Lipase / metabolism*
  • Lipolysis*
  • Male
  • Mice
  • Mice, Knockout
  • Muscle, Skeletal / metabolism
  • Perilipin-1
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • Protein Isoforms / genetics
  • Protein Isoforms / physiology
  • Proto-Oncogene Proteins c-jun / genetics
  • Proto-Oncogene Proteins c-jun / physiology*
  • Sterol Esterase / genetics
  • Sterol Esterase / metabolism*
  • Survival Analysis
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism


  • Carrier Proteins
  • Dietary Fats
  • Perilipin-1
  • Phosphoproteins
  • Protein Isoforms
  • Proto-Oncogene Proteins c-jun
  • Tumor Necrosis Factor-alpha
  • Sterol Esterase
  • Lipase
  • PNPLA2 protein, mouse