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. 2011 Jun 21;11(12):2042-4.
doi: 10.1039/c1lc20231f. Epub 2011 May 4.

Sensitive On-Chip Detection of a Protein Biomarker in Human Serum and Plasma Over an Extended Dynamic Range Using Silicon Photonic Microring Resonators and Sub-Micron Beads

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Free PMC article

Sensitive On-Chip Detection of a Protein Biomarker in Human Serum and Plasma Over an Extended Dynamic Range Using Silicon Photonic Microring Resonators and Sub-Micron Beads

Matthew S Luchansky et al. Lab Chip. .
Free PMC article

Abstract

We demonstrate a three-step assay on a silicon photonic microring resonator-based detection platform that enables the quantitation of the cardiac biomarker C-reactive protein (CRP) over a dynamic range spanning six orders of magnitude. Using antibody-modified microrings, we sequentially monitor primary CRP binding, secondary recognition of bound CRP by a biotinylated antibody, and tertiary signal amplification using streptavidin-functionalized beads. This detection methodology is applied to CRP quantitation in human serum and plasma samples.

Figures

Fig. 1
Fig. 1
Schematic and real-time data plot showing sequential addition of CRP, biotinylated secondary antibody, and SA-functionalized beads. The red trace is 10−1 μg/mL CRP. The blue trace is 10−3 μg/mL CRP. * indicates buffer rinse, and arrows indicate solution injection.
Fig. 2
Fig. 2
Log-log calibration plot showing the response of the microring resonators to varying concentrations of CRP using the three-step assay. Black squares indicate the initial slope of the primary binding (right axis), red circles indicate secondary antibody shift, and blue triangles indicate bead shift (left axis). Error bars represent 95% CI for n=17–47 rings for each concentration. Arrows at top represent overlap of dynamic ranges for each assay portion.
Fig. 3
Fig. 3
Detection of CRP in human serum and plasma samples. All samples are diluted 1:1000 in buffer except for the CRP-depleted serum, which was diluted 1:100. Error bars represent the error in the x-intercept determination used in the standard addition analysis.

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