Sensitive on-chip detection of a protein biomarker in human serum and plasma over an extended dynamic range using silicon photonic microring resonators and sub-micron beads

Lab Chip. 2011 Jun 21;11(12):2042-4. doi: 10.1039/c1lc20231f. Epub 2011 May 4.


We demonstrate a three-step assay on a silicon photonic microring resonator-based detection platform that enables the quantitation of the cardiac biomarker C-reactive protein (CRP) over a dynamic range spanning six orders of magnitude. Using antibody-modified microrings, we sequentially monitor primary CRP binding, secondary recognition of bound CRP by a biotinylated antibody, and tertiary signal amplification using streptavidin-functionalized beads. This detection methodology is applied to CRP quantitation in human serum and plasma samples.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biomarkers / analysis*
  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • C-Reactive Protein / chemistry*
  • Humans
  • Immunoassay / instrumentation
  • Immunoassay / methods
  • Particle Size
  • Plasma / chemistry*
  • Serum / chemistry*
  • Silicon / chemistry*


  • Biomarkers
  • C-Reactive Protein
  • Silicon