Human cytomegalovirus stimulates arachidonic acid metabolism through pathways that are affected by inhibitors of phospholipase A2 and protein kinase C

Biochem Biophys Res Commun. 1990 Jan 30;166(2):953-9. doi: 10.1016/0006-291x(90)90903-z.

Abstract

Inhibitors of phospholipase A2, tetracaine and quinacrine, inhibitors of protein kinases, H-7 and H-8, and a diacylglycerol lipase inhibitor reduced the level of CMV-induced [3H]AA release. A combination of H-7 and quinacrine inhibited stimulation of [3H]AA by about 80%. LU cells chronically treated with TPA and infected with CMV, had a reduced level of CMV-induced [3H]AA release and in the presence of quinacrine it was completely inhibited. These results suggest that CMV-induced stimulation of AA metabolism is mediated by pathways which are associated with activation of PLA2 and protein kinase C.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Arachidonic Acid
  • Arachidonic Acids / metabolism*
  • Cells, Cultured
  • Cytomegalovirus / physiology
  • Cytomegalovirus Infections / metabolism*
  • Diacylglycerol Kinase
  • Humans
  • In Vitro Techniques
  • Isoquinolines / pharmacology
  • Lipase / antagonists & inhibitors
  • Phospholipases / antagonists & inhibitors*
  • Phospholipases A / antagonists & inhibitors*
  • Phospholipases A2
  • Phosphotransferases / antagonists & inhibitors
  • Piperazines / pharmacology
  • Protein Kinase C / antagonists & inhibitors*
  • Quinacrine / pharmacology
  • Tetracaine / pharmacology
  • Tetradecanoylphorbol Acetate / pharmacology

Substances

  • Arachidonic Acids
  • Isoquinolines
  • Piperazines
  • Tetracaine
  • Arachidonic Acid
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide
  • Phosphotransferases
  • Diacylglycerol Kinase
  • Protein Kinase C
  • Phospholipases
  • Lipase
  • Phospholipases A
  • Phospholipases A2
  • Quinacrine
  • Tetradecanoylphorbol Acetate