Black tea and theaflavins suppress various inflammatory modulators and i-NOS mediated nitric oxide synthesis during gastric ulcer healing

Free Radic Res. 2011 Jul;45(7):767-78. doi: 10.3109/10715762.2011.579119. Epub 2011 May 5.

Abstract

The modulation of the cyclooxygenase-independent pathway by black tea (BT) and its constituent theaflavins (TFs) during their healing action against indomethacin-induced stomach ulceration in mice was investigated. On the 3(rd) day of its administration, indomethacin (18 mg/kg) induced maximum stomach ulceration, which was associated with increased myeloperoxidase (MPO) activity (93.3%, p<0.001), and inducible nitric oxide synthase (iNOS) expression (1.6-fold, p<0.001), along with augmented levels of serum nitrite (1.5-fold, p<0.001), selectins and cell adhesion molecules (CAMs), as well as reduced endothelial nitric oxide synthase (eNOS) expression (60%, p<0.001). Treatment with BT (40 mg/kg) and TF (1 mg/kg) for 3 days reversed these parameters and provided excellent (78-81%) ulcer healing. However, alterations of NOS expressions and levels of selectins and CAMs were only partially responsible for the excellent healing capacity (∼80%) of omeprazole (3 mg/kg × 3 days).

MeSH terms

  • Animals
  • Biflavonoids / therapeutic use*
  • Catechin / therapeutic use*
  • Cell Adhesion Molecules / blood
  • Immunoblotting
  • Indomethacin
  • Mice
  • Nitric Oxide / biosynthesis*
  • Nitric Oxide Synthase Type II / metabolism*
  • Nitric Oxide Synthase Type III / biosynthesis
  • Nitric Oxide Synthase Type III / blood
  • Nitric Oxide Synthase Type III / genetics
  • Nitrites / blood
  • Omeprazole / pharmacology
  • Peroxidase / metabolism
  • Selectins / blood
  • Stomach Ulcer / chemically induced
  • Stomach Ulcer / drug therapy*
  • Tea / chemistry*
  • Wound Healing / drug effects*

Substances

  • Biflavonoids
  • Cell Adhesion Molecules
  • Nitrites
  • Selectins
  • Tea
  • theaflavin
  • Nitric Oxide
  • Catechin
  • Peroxidase
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase Type III
  • Omeprazole
  • Indomethacin