Identification of a new membrane-associated polypeptide specified by the coronavirus infectious bronchitis virus

J Gen Virol. 1990 Jan:71 ( Pt 1):3-11. doi: 10.1099/0022-1317-71-1-3.

Abstract

Nucleotide sequences from the third open reading frame of mRNA D (D3) of infectious bronchitis virus (IBV) were expressed in bacteria as part of a fusion protein with beta-galactosidase. Antiserum raised in rabbits against this fusion protein immunoprecipitated from IBV-infected chick kidney or Vero cells a polypeptide of 12.4K, the size expected for a D3-encoded product. The D3 polypeptide is apparently non-glycosylated, and appears to be associated with the membrane fraction of infected cells, as judged by cell fractionation and immunofluorescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Viral / biosynthesis
  • Base Sequence
  • Cell Fractionation
  • Cell Membrane / analysis
  • Cloning, Molecular
  • Coronaviridae / genetics*
  • DNA, Viral / genetics
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Viral
  • Infectious bronchitis virus / analysis
  • Infectious bronchitis virus / genetics*
  • Infectious bronchitis virus / ultrastructure
  • Membrane Proteins / analysis
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Peptides / analysis
  • Peptides / genetics
  • Plasmids
  • Precipitin Tests
  • Protein Biosynthesis
  • RNA, Messenger / genetics*
  • RNA, Viral / analysis*
  • Transcription, Genetic
  • Vero Cells
  • Viral Fusion Proteins / biosynthesis
  • Viral Fusion Proteins / genetics
  • Viral Proteins / analysis
  • Viral Proteins / genetics*

Substances

  • Antibodies, Viral
  • DNA, Viral
  • Membrane Proteins
  • Peptides
  • RNA, Messenger
  • RNA, Viral
  • Viral Fusion Proteins
  • Viral Proteins