Abstract
Nucleotide sequences from the third open reading frame of mRNA D (D3) of infectious bronchitis virus (IBV) were expressed in bacteria as part of a fusion protein with beta-galactosidase. Antiserum raised in rabbits against this fusion protein immunoprecipitated from IBV-infected chick kidney or Vero cells a polypeptide of 12.4K, the size expected for a D3-encoded product. The D3 polypeptide is apparently non-glycosylated, and appears to be associated with the membrane fraction of infected cells, as judged by cell fractionation and immunofluorescence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Viral / biosynthesis
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Base Sequence
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Cell Fractionation
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Cell Membrane / analysis
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Cloning, Molecular
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Coronaviridae / genetics*
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DNA, Viral / genetics
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Fluorescent Antibody Technique
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Gene Expression Regulation, Viral
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Infectious bronchitis virus / analysis
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Infectious bronchitis virus / genetics*
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Infectious bronchitis virus / ultrastructure
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Membrane Proteins / analysis
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Membrane Proteins / genetics*
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Molecular Sequence Data
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Peptides / analysis
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Peptides / genetics
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Plasmids
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Precipitin Tests
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Protein Biosynthesis
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RNA, Messenger / genetics*
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RNA, Viral / analysis*
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Transcription, Genetic
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Vero Cells
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Viral Fusion Proteins / biosynthesis
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Viral Fusion Proteins / genetics
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Viral Proteins / analysis
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Viral Proteins / genetics*
Substances
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Antibodies, Viral
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DNA, Viral
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Membrane Proteins
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Peptides
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RNA, Messenger
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RNA, Viral
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Viral Fusion Proteins
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Viral Proteins