Identification and characterization of TcCRP1, a pollen tube attractant from Torenia concolor

Ann Bot. 2011 Sep;108(4):739-47. doi: 10.1093/aob/mcr111. Epub 2011 May 5.


Background and aims: During sexual reproduction in higher angiosperms, the pollen tubes are directed to the ovules in the pistil to deliver sperm cells. This pollen tube attraction is highly species specific, and a group of small secreted proteins, TfCRPs, are necessary for this process in Torenia fournieri.

Methods: A candidate pollen tube attractant protein in Torenia concolor, a related species of T. fournieri, was isolated and the attractant abilities between them were compared.

Key results: TcCRP1, an orthologous gene of TfCRP1 from T. concolor, is expressed predominantly in the synergid cell. The gene product attracted pollen tubes in a concentration-dependent manner, but attracted fewer pollen tubes from the other species.

Conclusions: The results indicated that this class of CRP proteins is a common pollen tube attractant in Torenia species. The sequence diversity of these proteins is important for species-specific pollen tube attraction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Crosses, Genetic
  • Gene Expression Regulation, Plant
  • Lamiaceae / cytology
  • Lamiaceae / genetics
  • Lamiaceae / metabolism*
  • Lamiaceae / physiology
  • Molecular Sequence Data
  • Organ Specificity / genetics
  • Ovule / cytology
  • Ovule / physiology
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / isolation & purification
  • Plant Proteins / metabolism*
  • Pollen Tube / anatomy & histology
  • Pollen Tube / cytology
  • Pollen Tube / growth & development
  • Pollen Tube / metabolism*
  • Sequence Homology, Amino Acid
  • Species Specificity


  • Plant Proteins

Associated data

  • GENBANK/AB594190