MicroRNA-143 functions as a tumor suppressor in human bladder cancer T24 cells

Cancer Lett. 2011 Aug 28;307(2):211-20. doi: 10.1016/j.canlet.2011.04.005. Epub 2011 May 6.


MicroRNA (miR)-143 and -145 were down-regulated in human bladder cancer T24 cells. The enforced expression of miR-143 induced growth-suppression in T24 cells through down-regulation of ERK5 and Akt expression at translational level, and chemically-modified synthetic miR-143 (miR-143/BP) exhibited a greater growth inhibitory effect than wild-type miR-143. In addition, the synthetic miR-143/BP induced apoptotic cell death in some of the transfected cells. Furthermore, co-treatment with the synthetic miR-143/BP and cisplatin showed the additive growth-suppressing effect on T24 cells. These findings suggest that the chemically-modified synthetic miR-143 functions as a tumor suppressor in T24 cells by targeting ERK5 and/or Akt.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Western
  • Cell Line, Tumor
  • DNA Primers
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Urinary Bladder Neoplasms / pathology*


  • DNA Primers
  • MIRN143 microRNA, human
  • MicroRNAs