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Comparative Study
. 2011 Aug;88(8):1005-9.
doi: 10.1097/OPX.0b013e31821ddc6c.

Membrane Array Analysis of Tear Proteins in Ocular Cicatricial Pemphigoid

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Free PMC article
Comparative Study

Membrane Array Analysis of Tear Proteins in Ocular Cicatricial Pemphigoid

Matilda F Chan et al. Optom Vis Sci. .
Free PMC article

Abstract

Purpose: To explore non-invasive, protein-based, membrane array technology as a means to evaluate the global immune and angiogenic profile of tear proteins in patients with active ocular cicatricial pemphigoid (OCP).

Methods: Forty-three proteins consisting of cytokines, angiogenic/growth factors, and immunoinflammatory modulators were measured by membrane array in tear samples of four control patients and four OCP patients during active disease and after treatment.

Results: Signals for several distinct and consistent molecular entities were upregulated in all four active OCP tear samples relative to controls. In particular, interleukin-8 and matrix metalloproteinase-9 were elevated during active disease and decreased after systemic immunomodulatory therapy.

Conclusions: Protein array analysis may provide a well-tolerated assay to monitor levels of inflammatory markers in the tears of OCP patients in response to therapy.

Figures

Figure 1
Figure 1
Duplicate dots of capture antibodies specific for each protein as well as positive (pos) and negative (neg) controls are shown for a representative normal (normal 3) and OCP patient (OCP 3). Low to moderate signals for angiogenin, growth related oncogene (GRO), IL-6, IL-8, TIMP-1 and TIMP-2, MMP1, and MMP9 are detected in normal tears. Tears from the OCP patient were collected at the time of presentation (Pre) and after 4 and 8 weeks of immuno-modulatory therapy (IMT). Boxes denote the decrease in signal for IL-6, IL-8, and MMP-9 with treatment.
Figure 2
Figure 2
Graphical comparison of background-subtracted densitometric values (Y-axis) for tear proteins measured in four normal (N) patients and four OCP patients before and after treatment.

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