Membrane array analysis of tear proteins in ocular cicatricial pemphigoid

Optom Vis Sci. 2011 Aug;88(8):1005-9. doi: 10.1097/OPX.0b013e31821ddc6c.

Abstract

Purpose: To explore non-invasive, protein-based, membrane array technology as a means to evaluate the global immune and angiogenic profile of tear proteins in patients with active ocular cicatricial pemphigoid (OCP).

Methods: Forty-three proteins consisting of cytokines, angiogenic/growth factors, and immunoinflammatory modulators were measured by membrane array in tear samples of four control patients and four OCP patients during active disease and after treatment.

Results: Signals for several distinct and consistent molecular entities were upregulated in all four active OCP tear samples relative to controls. In particular, interleukin-8 and matrix metalloproteinase-9 were elevated during active disease and decreased after systemic immunomodulatory therapy.

Conclusions: Protein array analysis may provide a well-tolerated assay to monitor levels of inflammatory markers in the tears of OCP patients in response to therapy.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Biopsy
  • Conjunctiva / pathology
  • Enzyme-Linked Immunosorbent Assay
  • Eye Proteins / analysis*
  • Eye Proteins / immunology
  • Follow-Up Studies
  • Humans
  • Middle Aged
  • Pemphigoid, Benign Mucous Membrane / immunology
  • Pemphigoid, Benign Mucous Membrane / metabolism*
  • Pemphigoid, Benign Mucous Membrane / pathology
  • Prospective Studies
  • Protein Array Analysis / methods*
  • Reproducibility of Results
  • Severity of Illness Index
  • Tears / chemistry*
  • Tears / immunology

Substances

  • Eye Proteins
  • tear proteins