Primary structure of the S peplomer gene of bovine coronavirus and surface expression in insect cells

J Gen Virol. 1990 Feb:71 ( Pt 2):263-70. doi: 10.1099/0022-1317-71-2-263.

Abstract

The nucleotide sequence of the S peplomer gene of bovine coronavirus (BCV) has been determined. A single open reading frame of 4089 nucleotides encodes a polypeptide of 150K with 20 potential sites for addition of N-linked oligosaccharides. Expression of the cloned BCV S gene by a recombinant of Autographa californica nuclear polyhedrosis virus resulted in production of a 180K glycosylated polypeptide which was transported to the surface of the cell. Comparison of the BCV S gene with the analogous genes of murine hepatitis viruses shows that the BCV S polypeptide contains a unique domain of 138 amino acids not present in murine hepatitis virus strain JHM, but which has a partially homologous counterpart in strain A59. This domain accounts for most of the differences in size of the S gene products of these coronaviruses.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Cloning, Molecular
  • Coronaviridae / genetics*
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Viral
  • Glycoproteins / genetics*
  • Insect Viruses / genetics
  • Molecular Sequence Data
  • Moths
  • Murine hepatitis virus / genetics
  • Precipitin Tests
  • RNA, Viral / genetics*
  • Viral Envelope Proteins / genetics*

Substances

  • Glycoproteins
  • RNA, Viral
  • Viral Envelope Proteins

Associated data

  • GENBANK/D00662