NMR assignment and secondary structure of the C-terminal DNA binding domain of Arabidopsis thaliana VERNALIZATION1

Biomol NMR Assign. 2012 Apr;6(1):5-8. doi: 10.1007/s12104-011-9313-6. Epub 2011 May 8.


VERNALIZATION1 (VRN1) is a multidomain DNA binding protein from Arabidopsis thaliana that is required for the acceleration of flowering time in response to prolonged cold treatment; a physiological process called vernalization. VRN1 is a 39 kDa protein comprised of two B3 domains flanking a putative nuclear localization sequence and two PEST domains. Here we report the (1)H, (13)C and (15)N resonance assignments of the 134 residue C-terminal region of VRN1, comprising a B3 DNA binding domain of the REM family and an upstream region that is highly conserved among VRN1 homologs from other dicotyledonous plant species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis Proteins / chemistry*
  • Arabidopsis Proteins / metabolism*
  • Arabidopsis*
  • Conserved Sequence
  • DNA / metabolism*
  • Nuclear Magnetic Resonance, Biomolecular*
  • Protein Structure, Tertiary
  • Repressor Proteins / chemistry*
  • Repressor Proteins / metabolism*


  • Arabidopsis Proteins
  • Repressor Proteins
  • VRN1 protein, Arabidopsis
  • DNA