Antioxidant activity of eugenol: a structure-activity relationship study

J Med Food. 2011 Sep;14(9):975-85. doi: 10.1089/jmf.2010.0197. Epub 2011 May 9.

Abstract

Eugenol (4-allyl-2-methoxyphenol), a major phenolic component from clove oil (Eugenia caryophyllata), has several biological activities. To estimate the capacity of eugenol to act as an antioxidant, the following were studied: 1,1-diphenyl-2-picryl-hydrazyl-, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)-, and N,N-dimethyl-p-phenylenediamine-scavenging activity; total antioxidant activity; and ability to reduce ferric ions and cupric ions. Eugenol inhibited 96.7% (r(2)=0.9319) lipid peroxidation of a linoleic acid emulsion at a 15-μg/mL concentration. Butylated hydroxyanisole, butylated hydroxytoluene, α-tocopherol, and Trolox(®) displayed 95.4% (r(2)=0.8482), 99.7% (r(2)=0.7798), 84.6% (r(2)=0.9272), and 95.6% (r(2)=0.8511) inhibition of peroxidation, respectively, at the 15-μg/mL concentration. According to the results of this study, eugenol had the most powerful antioxidant activity and radical-scavenging activity. This study should prompt further studies of the antioxidant properties of eugenol.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Clove Oil / chemistry
  • Eugenol / chemistry
  • Eugenol / pharmacology*
  • Food Preservatives / chemistry
  • Food Preservatives / pharmacology
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / pharmacology
  • Kinetics
  • Lipid Peroxides / antagonists & inhibitors
  • Lipid Peroxides / chemistry
  • Oxidation-Reduction
  • Structure-Activity Relationship

Substances

  • Antioxidants
  • Clove Oil
  • Food Preservatives
  • Free Radical Scavengers
  • Lipid Peroxides
  • Eugenol