Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011;2:310.
doi: 10.1038/ncomms1301.

An Evolutionarily Conserved Three-Dimensional Structure in the Vertebrate Irx Clusters Facilitates Enhancer Sharing and Coregulation

Affiliations

An Evolutionarily Conserved Three-Dimensional Structure in the Vertebrate Irx Clusters Facilitates Enhancer Sharing and Coregulation

Juan J Tena et al. Nat Commun. .

Abstract

Developmental gene clusters are paradigms for the study of gene regulation; however, the mechanisms that mediate phenomena such as coregulation and enhancer sharing remain largely elusive. Here we address this issue by analysing the vertebrate Irx clusters. We first present a deep enhancer screen of a 2-Mbp span covering the IrxA cluster. Using chromosome conformation capture, we show that enhancer sharing is widespread within the cluster, explaining its evolutionarily conserved organization. We also identify a three-dimensional architecture, probably formed through interactions with CCCTC-binding factor, which is present within both Irx clusters of mouse, Xenopus and zebrafish. This architecture brings the promoters of the first two genes together in the same chromatin landscape. We propose that this unique and evolutionarily conserved genomic architecture of the vertebrate Irx clusters is essential for the coregulation of the first two genes and simultaneously maintains the third gene in a partially independent regulatory landscape.

Similar articles

See all similar articles

Cited by 33 articles

See all "Cited by" articles

References

    1. EMBO J. 1998 Mar 16;17(6):1788-98 - PubMed
    1. Nat Protoc. 2007;2(7):1722-33 - PubMed
    1. Dev Genes Evol. 2001 May;211(5):257-60 - PubMed
    1. Mol Cell. 2002 Dec;10(6):1453-65 - PubMed
    1. EMBO J. 1998 Jan 2;17(1):191-203 - PubMed

Publication types

LinkOut - more resources

Feedback