Induction of adaptive response in human blood lymphocytes exposed to 900 MHz radiofrequency fields: influence of cell cycle

Int J Radiat Biol. 2011 Sep;87(9):993-9. doi: 10.3109/09553002.2011.574779. Epub 2011 May 11.


Purpose: To investigate the influence of cell cycle on the adaptive response (AR) induced by the exposure of human blood lymphocytes to radiofrequency fields (RF).

Materials and methods: Human peripheral blood lymphocytes in G(0)-, G(1)- or S-phase of the cell cycle were exposed for 20 hours to an adaptive dose (AD) of 900 MHz RF at an average specific absorption rate of 1.25 W/kg and then treated with a challenge dose (CD) of 100 ng/ml mitomycin C (MMC). Un-exposed and sham-exposed controls as well as cells treated with MMC alone were included in the study. The incidence of micronuclei (MN) was evaluated to determine the induction of AR.

Results: The results indicated that the cells which were exposed to AD of RF in G(0)- and G(1)-phase of the cell cycle did not exhibit AR while such a response was observed when the cells were exposed to AD of RF in S-phase of the cell cycle.

Conclusions: These results confirmed the observations reported in our previous investigation where AR was observed in human blood lymphocytes exposed to AD of RF in S-phase of the cell cycle and further suggested that the timing of AD exposure of RF is important to elicit AR.

MeSH terms

  • Adaptation, Physiological / drug effects
  • Adaptation, Physiological / genetics
  • Adaptation, Physiological / radiation effects*
  • Adult
  • Cell Cycle / drug effects
  • Cell Cycle / genetics
  • Cell Cycle / radiation effects*
  • Cell Proliferation / drug effects
  • Cell Proliferation / radiation effects
  • Dose-Response Relationship, Drug
  • Dose-Response Relationship, Radiation
  • Humans
  • Lymphocytes / cytology*
  • Lymphocytes / drug effects
  • Lymphocytes / metabolism
  • Lymphocytes / radiation effects*
  • Male
  • Micronucleus Tests
  • Middle Aged
  • Mitomycin / pharmacology
  • Radio Waves / adverse effects*
  • Time Factors
  • Young Adult


  • Mitomycin