Protein recognition sites in polyomavirus enhancer: formation of a novel site for NF-1 factor in an enhancer mutant and characterization of a site in the enhancer D domain

EMBO J. 1990 Mar;9(3):947-55.

Abstract

Polyomavirus mutants selected for modified host range exhibit DNA sequence alterations in the regulatory region, which consist mainly of duplications and/or deletions. Single base pair mutations have also been observed, which create or abolish DNA sequence motifs recognized by DNA-binding regulatory factors. The present work deals with the molecular characterization of a Polyoma mutant (PyNB11/1), selected for its high efficiency of growth in neuroblastoma cells. The enhancer region of PyNB11/1 displays a 91 bp tandem duplication harbouring a novel DNA sequence motif created at the boundary of the duplicated fragment. This motif is absent in the wild-type enhancer and is specifically recognized by a nuclear factor that belongs to the NF-1 family of transcription factors. We also report the characterization of an as yet unidentified DNA sequence motif in the D domain of the viral enhancer, that represents the binding site for a nuclear factor that is ubiquitous and comparably abundant in several murine cell types.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • DNA, Viral / genetics
  • DNA, Viral / metabolism
  • Deoxyribonuclease I
  • Enhancer Elements, Genetic*
  • Genes, Regulator*
  • Mice
  • Molecular Sequence Data
  • Mutation*
  • Neuroblastoma
  • Oligonucleotide Probes
  • Polyomavirus / genetics*
  • Restriction Mapping
  • Transcription Factors / metabolism*

Substances

  • DNA, Viral
  • Oligonucleotide Probes
  • Transcription Factors
  • Deoxyribonuclease I