Topostin, a novel inhibitor of mammalian DNA topoisomerase I from Flexibacter topostinus sp. nov. I. Taxonomy, and fermentation of producing strain

J Antibiot (Tokyo). 1990 Feb;43(2):154-7. doi: 10.7164/antibiotics.43.154.

Abstract

We found a new inhibitor of mammalian DNA topoisomerase I, named topostin, from a bacterial culture. The bacteria was identified as Flexibacter topostinus sp. nov., B-572. Morphological and physiological characteristics, and utilization of sugars were examined. Comparison of the strain with known species of the genus Flexibacter was made and indicates that the strain is a new species of the genus Flexibacter. The bacteria produced the inhibitor in parallel with their growth up to 72 hours.

MeSH terms

  • Culture Media / analysis
  • Cytophaga / metabolism*
  • Cytophaga / ultrastructure
  • DNA Topoisomerases, Type I / isolation & purification
  • DNA Topoisomerases, Type I / pharmacology
  • Enzyme Inhibitors / isolation & purification*
  • Fermentation
  • Organic Chemicals*
  • Topoisomerase I Inhibitors*

Substances

  • Culture Media
  • Enzyme Inhibitors
  • Organic Chemicals
  • Topoisomerase I Inhibitors
  • topostin A1
  • topostin A2
  • topostin B
  • DNA Topoisomerases, Type I