Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

Chem Commun (Camb). 2011 Jul 14;47(26):7503-5. doi: 10.1039/c1cc11953b. Epub 2011 May 11.

Abstract

Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).

MeSH terms

  • Biocatalysis*
  • Bordetella bronchiseptica / enzymology
  • Carboxy-Lyases / chemistry*
  • Carboxy-Lyases / genetics
  • Carboxy-Lyases / metabolism*
  • Catalytic Domain
  • Directed Molecular Evolution / methods*
  • Models, Molecular
  • Mutation
  • Substrate Specificity
  • Sulfur / metabolism*

Substances

  • Sulfur
  • Carboxy-Lyases
  • malonate decarboxylase