Abstract
Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).
MeSH terms
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Biocatalysis*
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Bordetella bronchiseptica / enzymology
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Carboxy-Lyases / chemistry*
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Carboxy-Lyases / genetics
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Carboxy-Lyases / metabolism*
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Catalytic Domain
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Directed Molecular Evolution / methods*
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Models, Molecular
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Mutation
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Substrate Specificity
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Sulfur / metabolism*
Substances
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Sulfur
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Carboxy-Lyases
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malonate decarboxylase