Establishment and characterization of pthrp-producing human pancreatic-cancer cell-line

Y Nishi; M Haji; R Takayanagi; H Iguchi; T Shimazoe; J Hirata; H Nawata

Establishment and characterization of pthrp-producing human pancreatic-cancer cell-line

Int J Oncol. 1994 Jul;5(1):33-9.

Authors

Y Nishi¹, M Haji, R Takayanagi, H Iguchi, T Shimazoe, J Hirata, H Nawata

Affiliation

¹ NATL KYUSHU CANC CTR,CHEMOTHERAPY DIV RES INST,MINAMI KU,FUKUOKA 815,JAPAN.

PMID: 21559554

Abstract

Human pancreatic ductal cell carcinoma cell line, which can secrete parathyroid hormone-related peptide (PTHrP), designated as KP 4, and its daughter cell lines with different PTHrP-secreting activities, termed KP 4-1 and KP 4-2, have been established in tissue culture. KP 4 cells were able to form tumors in nude mice. The absolute production rate of PTHrP in KP 4-1 was 5 to 10 times higher than that in KP 4-2. Similarly, the level of PTHrP mRNA in KP 4-1 was significantly higher than that in KP 4-2. KP 4-2 cells exhibited more rapid growth than KP 4 and KP 4-1 in vitro. Our established cell lines should provide a useful system to study the regulation of PTHrP production and its pathophysiological roles.