The glutamate receptor of the Qp-type activates protein kinase C and is regulated by protein kinase C

Neurosci Lett. 1990 Feb 5;109(1-2):146-51. doi: 10.1016/0304-3940(90)90553-l.


In striatal neurons in primary culture quisqualate potently stimulated the formation of inositol phosphates via a metabotropic receptor we recently termed Qp in order to distinguish it from the classical ionotropic quisqualate receptor termed Qi. Here we show that 10 microM of quisqualate activated in a rapid and transient manner protein kinase C as assessed by its translocation from the cytosolic to the membrane fraction. As 10 microM alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA), the Qi specific agonist, was without effect, this translocation was most probably mediated by the Qp receptor. Phorbol 12,13-dibutyrate blocked in a dose-dependent manner the Qp receptor-induced inositol phosphate formation (IC50 = 2 +/- 0.4 nM). The inactive ester 4 alpha-phorbol-12,13-didecanoate was without effect. Very low concentrations of staurosporine completely reversed the phorbol 12,13-dibutyrate-induced blockade (IC50 = 2.2 +/- 1.3 nM). It can therefore be concluded that the Qp receptor is able to activate protein kinase C and that the activity of this metabotropic receptor is regulated by protein kinase C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / pharmacology
  • Animals
  • Corpus Striatum / drug effects
  • Corpus Striatum / metabolism*
  • Cytosol / enzymology
  • Ibotenic Acid / analogs & derivatives
  • Ibotenic Acid / pharmacology
  • Phorbol 12,13-Dibutyrate / pharmacology
  • Protein Kinase C / metabolism*
  • Protein Kinase C / physiology
  • Receptors, AMPA
  • Receptors, Neurotransmitter / drug effects
  • Receptors, Neurotransmitter / physiology*
  • Staurosporine
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid


  • Alkaloids
  • Receptors, AMPA
  • Receptors, Neurotransmitter
  • Ibotenic Acid
  • Phorbol 12,13-Dibutyrate
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
  • Protein Kinase C
  • Staurosporine