PPARα is regulated by miR-21 and miR-27b in human liver

Pharm Res. 2011 Oct;28(10):2467-76. doi: 10.1007/s11095-011-0473-y. Epub 2011 May 12.


Purpose: Peroxisome proliferator-activated receptor α (PPARα) is an important transcriptional factor that regulates genes encoding endo/xenobiotic enzymes and lipid metabolizing enzymes. In this study, we investigated whether microRNAs (miRNAs) are involved in the regulation of PPARα in human liver.

Methods: Precursor or antisense oligonucleotide for miR-21 or miR-27b was transfected into HuH7 cells; expression of PPARα and acyl-CoA synthetase M2B was determined by Western blot and real-time RT-PCR. Luciferase assay was performed to identify the functional miRNA recognition element (MRE). Expression levels of PPARα, miR-21, and miR-27b in a panel of 24 human livers were determined.

Results: The overexpression and inhibition of miR-21 or miR-27b in HuH7 cells significantly decreased and increased the PPARα protein level, respectively, but not PPARα mRNA level. The miRNA-dependent regulation of PPARα affected the expression of its downstream gene. Luciferase assay identified a functional MRE for miR-21 in the 3'-untranslated region of PPARα. In human livers, the PPARα protein levels were not correlated with PPARα mRNA, but inversely correlated with the miR-21 levels, suggesting a substantial impact of miR-21, although the contribution of miR-27b could not be ruled out.

Conclusions: We found that PPARα in human liver is regulated by miRNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Cell Line, Transformed
  • Cell Line, Tumor
  • Gene Expression Regulation*
  • HEK293 Cells
  • HeLa Cells
  • Hep G2 Cells
  • Humans
  • Liver / metabolism
  • Liver / physiology*
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • PPAR alpha / genetics*
  • PPAR alpha / metabolism
  • RNA, Messenger / genetics
  • Transfection


  • 3' Untranslated Regions
  • MIRN21 microRNA, human
  • MIRN27 microRNA, human
  • MicroRNAs
  • PPAR alpha
  • RNA, Messenger