Characterization of 12 polymorphic microsatellite markers for Georgia false indigo (Amorpha georgiana Wilbur var. georgiana), an endangered species, and their utility in other dwarf Amorpha L. species

Shannon C K Straub; Steven M Bogdanowicz; Jeff J Doyle

doi:10.1111/j.1755-0998.2008.02409.x

Characterization of 12 polymorphic microsatellite markers for Georgia false indigo (Amorpha georgiana Wilbur var. georgiana), an endangered species, and their utility in other dwarf Amorpha L. species

Mol Ecol Resour. 2009 Jan;9(1):225-8. doi: 10.1111/j.1755-0998.2008.02409.x. Epub 2008 Oct 21.

Authors

Shannon C K Straub¹, Steven M Bogdanowicz, Jeff J Doyle

Affiliation

¹ L.H. Bailey Hortorium, Department of Plant Biology, Cornell University, 412 Mann Library, Ithaca, NY 14853, USA, Evolutionary Genetics Core Facility, Department of Ecology and Evolutionary Biology, Cornell University, E344 Corson Hall, Ithaca, NY 14853, USA.

PMID: 21564610
DOI: 10.1111/j.1755-0998.2008.02409.x

Abstract

In order to facilitate the addition of a genetic component to conservation management plans for Georgia false indigo (Amorpha georgiana var. georgiana), a rare legume of the southeastern USA, 12 polymorphic microsatellite markers were developed. No gametic disequilibrium was detected among locus pairs, but observations for five of the loci significantly deviated from expected Hardy-Weinberg proportions. Cross-species testing was successful and demonstrated the utility of the majority of the markers in congeners Amorpha georgiana var. confusa and Amorpha herbacea. The results also suggested that A. georgiana var. confusa is tetraploid rather than diploid.