A two-state stabilization-change mechanism for proton-pumping complex I

Biochim Biophys Acta. 2011 Oct;1807(10):1364-9. doi: 10.1016/j.bbabio.2011.04.006. Epub 2011 May 2.


Despite its central function in oxidative phosphorylation, the molecular mechanism of proton pumping respiratory complex I is still elusive. In recent years, considerable progress has been made towards understanding structure/function relationships in this very large and complicated membrane protein complex. Last year X-ray crystallographic analysis of bacterial and mitochondrial complex I provided important insights into its molecular architecture. Based on this evidence, here a hypothetical molecular mechanism for redox-driven proton pumping of complex I is proposed. According to this mechanism, two pump modules are driven by two conformational strokes that are generated by stabilization of the anionic forms of semiquinone and ubiquinol that are formed in the peripheral arm of complex I during turnover. This results in the experimentally determined pumping stoichiometry of 4 H(+)/2e(-). In the two-state model, electron transfer from iron-sulfur cluster N2 is allowed only in the 'E-state,' while protonation of the substrate is only possible in the stabilizing 'P-state.' In the membrane arm, transition from the E- to the P-state drives the two pump modules via long range conformational energy transfer through the recently discovered helical transmission element connecting them. The proposed two-state stabilization-change mechanism is fully reversible and thus inherently explains the operation of complex I in forward and reverse mode. This article is part of a Special Issue entitled Allosteric cooperativity in respiratory proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / metabolism
  • Benzoquinones / chemistry
  • Benzoquinones / metabolism
  • Binding Sites
  • Crystallography, X-Ray
  • Electron Transport
  • Electron Transport Complex I / chemistry*
  • Electron Transport Complex I / metabolism
  • Iron-Sulfur Proteins / chemistry
  • Iron-Sulfur Proteins / metabolism
  • Mitochondrial Membranes / metabolism
  • Mitochondrial Proteins / chemistry
  • Mitochondrial Proteins / metabolism
  • Models, Biological
  • Models, Chemical*
  • Models, Molecular
  • Molecular Structure
  • Oxidation-Reduction
  • Oxidative Phosphorylation
  • Protein Binding
  • Protein Conformation
  • Protein Structure, Secondary
  • Proton Pumps / chemistry*
  • Proton Pumps / metabolism
  • Protons*


  • Bacterial Proteins
  • Benzoquinones
  • Iron-Sulfur Proteins
  • Mitochondrial Proteins
  • Proton Pumps
  • Protons
  • quinone
  • Electron Transport Complex I