ARHGAP30 is a Wrch-1-interacting protein involved in actin dynamics and cell adhesion

Biochem Biophys Res Commun. 2011 May 27;409(1):96-102. doi: 10.1016/j.bbrc.2011.04.116. Epub 2011 May 1.


The atypical Rho GTPase Wrch-1 has been proposed roles in cell migration, focal adhesion dissolution, stress fibre break down and tight junction heterogeneity. A screen for Wrch-1 binding-partners identified the novel RhoGAP protein, ARHGAP30, as a Wrch-1 interactor. ARHGAP30 is related to the Cdc42- and Rac1-specific RhoGAP CdGAP, which was likewise found to bind Wrch-1. In contrast to CdGAP, ARHGAP30 serves as a Rac1- and RhoA-specific RhoGAP. Ectopic expression of ARHGAP30 results in membrane blebbing and dissolution of stress-fibres and focal adhesions. Our data suggest roles for ARHGAP30 and CdGAP in regulation of cell adhesion downstream of Wrch-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Animals
  • Cell Adhesion
  • Cells, Cultured
  • GTPase-Activating Proteins / classification
  • GTPase-Activating Proteins / genetics
  • GTPase-Activating Proteins / metabolism*
  • HEK293 Cells
  • Humans
  • Mice
  • Phylogeny
  • Swine
  • rho GTP-Binding Proteins / genetics
  • rho GTP-Binding Proteins / metabolism*


  • ARHGAP30 protein, human
  • Actins
  • GTPase-Activating Proteins
  • RHOU protein, human
  • rho GTP-Binding Proteins