Cloning, localization, and permanent expression of a Drosophila octopamine receptor

Neuron. 1990 Mar;4(3):343-54. doi: 10.1016/0896-6273(90)90047-j.


A cDNA for a member of the G protein-coupled receptor family was isolated from Drosophila using a probe derived from a human beta 2-adrenergic receptor cDNA. This Drosophila receptor gene is localized at 99A10-B1 on the right arm of chromosome 3 and is preferentially expressed in Drosophila heads. The insect octopamine receptor has been permanently expressed in mammalian cells, where it mediates the attenuation of adenylate cyclase activity and exhibits a pharmacological profile consistent with an octopamine type 1 receptor. Sequence and pharmacological comparisons indicate that the octopamine receptor is unique but closely related to mammalian adrenergic receptors, perhaps as an evolutionary precursor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism
  • Cell Line
  • Chromosome Mapping
  • Cloning, Molecular / methods
  • DNA / genetics
  • DNA / isolation & purification
  • Drosophila / genetics*
  • GTP-Binding Proteins / genetics
  • Gene Expression*
  • Genes*
  • Genomic Library
  • Humans
  • Molecular Sequence Data
  • Octopamine / metabolism
  • Oligonucleotide Probes
  • Protein Conformation
  • Receptors, Adrenergic / genetics*
  • Receptors, Adrenergic, beta / genetics
  • Receptors, Biogenic Amine*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transfection


  • Oligonucleotide Probes
  • Receptors, Adrenergic
  • Receptors, Adrenergic, beta
  • Receptors, Biogenic Amine
  • norsynephrine receptor
  • Octopamine
  • DNA
  • GTP-Binding Proteins

Associated data

  • GENBANK/M26181