Quantification of growth and differentiation of C2C12 skeletal muscle cells on PSS-PAH-based polyelectrolyte layer-by-layer nanofilms

Biomed Mater. 2011 Jun;6(3):031001. doi: 10.1088/1748-6041/6/3/031001. Epub 2011 May 13.


Polyelectrolyte layer-by-layer (LbL) nanofilms are interesting polymeric structures, built by alternating adsorption of positively and negatively charged polyelectrolytes. They consist of multilayer sheets with nanometric overall thickness, and they can be used as supports and surface coatings for in vitro and in vivo cell and tissue growth and regeneration. The present study focuses on nanofilms based on alternated layers of poly(sodium-4-sulfonate) (PSS) and poly(allylamine hydrochloride) (PAH) fabricated using spin-assisted LbL assembly (SA-LbL). The fabrication process used to assemble polyelectrolyte nanofilms made of up to 60 bilayers is described, and the influence of different surface charges (i.e. changing the terminal layer) and of different film composition (e.g. varying PSS molecular weight) on cell behaviour is investigated. In particular, C2C12 skeletal muscle cells' viability, proliferation and differentiation on six different typologies of polyelectrolyte nanofilms are evaluated and quantified, giving a reference for skeletal muscle regeneration capabilities on such kind of structures.

MeSH terms

  • Actins / metabolism
  • Animals
  • Biocompatible Materials / chemistry*
  • Cell Differentiation
  • Cell Line
  • Cell Proliferation
  • Cell Survival
  • Electrolytes / chemistry*
  • Materials Testing
  • Mice
  • Muscle, Skeletal / cytology*
  • Nanostructures / chemistry
  • Polyamines / chemistry*
  • Polymers / chemistry*
  • Silicon / chemistry
  • Sulfones / chemistry*
  • Sulfonic Acids / chemistry*


  • Actins
  • Biocompatible Materials
  • Electrolytes
  • Polyamines
  • Polymers
  • Sulfones
  • Sulfonic Acids
  • polyallylamine
  • Silicon
  • styrenesulfonic acid polymer