G-quadruplex RNA structure as a signal for neurite mRNA targeting

EMBO Rep. 2011 Jul 1;12(7):697-704. doi: 10.1038/embor.2011.76.

Abstract

Targeting of messenger RNAs (mRNAs) in neuron processes relies on cis-acting regulatory elements, the nature of which is poorly understood. Here, we report that approximately 30% of the best-known dendritic mRNAs contain a guanine (G)-quadruplex consensus in their 3'-untranslated region. Among these mRNAs, we show by using RNA structure probing that a G-quadruplex is present in the mRNAs of two key postsynaptic proteins: PSD-95 and CaMKIIa. The G-quadruplex structure is necessary and sufficient for the potent and fast localization of mRNAs in cortical neurites and this occurs in a metabotropic glutamate receptor-responsive manner. Thus, G-quadruplex seems to be a common neurite localization signal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2 / genetics
  • Disks Large Homolog 4 Protein
  • G-Quadruplexes*
  • Humans
  • Intracellular Signaling Peptides and Proteins / genetics
  • Membrane Proteins / genetics
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Neurites / metabolism*
  • Nucleic Acid Conformation*
  • RNA Transport
  • RNA, Messenger / chemistry*
  • RNA, Messenger / metabolism*

Substances

  • DLG4 protein, human
  • Disks Large Homolog 4 Protein
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • RNA, Messenger
  • CAMK2A protein, human
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2