Biological effects and binding properties of transforming growth factor-beta on human oral squamous cell carcinoma cells

Exp Cell Res. 1990 Apr;187(2):263-9. doi: 10.1016/0014-4827(90)90090-w.


The effects of transforming growth factor-beta (TGF-beta) on three human oral squamous cell carcinoma cell lines, HSC-2, HSC-3, and HSC-4, were investigated. Although these cell lines were equally sensitive to epidermal growth factor, responses to TGF-beta were variable. Dose-dependent inhibition of cell growth and [3H]thymidine incorporation of HSC-4 were observed by the addition of TGF-beta, whereas growth inhibitory effects on HSC-2 and HSC-3 were marginal. Moreover, treatment of HSC-4 with TGF-beta led to a more than 300-fold increase in fibronectin secretion into the medium. In contrast, TGF-beta did not increase the secretion of fibronectin on HSC-2 and HSC-3. Scatchard analysis of the binding of TGF-beta suggested that all squamous cell carcinoma cell lines have similar binding properties, with two classes of binding sites for TGF-beta. Affinity labeling of 125I-TGF-beta to cell surface receptors revealed the two major affinity crosslinked bands with Mr values of 65 kDa (type I) and 280 kDa (type III). A concomitant loss of 85 kDa band (type II) was observed in all squamous carcinoma cell lines examined. Although the proportions of type I and type III receptors were variable, the type I receptor, which is reported to be the main functional receptor in mediating the TGF-beta action, was commonly observed in these squamous cell carcinoma cell lines. These results indicate that the heterogeneity in response to TGF-beta between cell lines may be due to the difference in the signal transduction pathway of TGF-beta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Squamous Cell
  • Cell Division / drug effects
  • Cell Line
  • Cross-Linking Reagents / metabolism
  • DNA Replication / drug effects
  • Fibronectins / analysis
  • Fibronectins / biosynthesis
  • Humans
  • Immunoenzyme Techniques
  • Kinetics
  • Mouth Neoplasms
  • Receptors, Cell Surface / metabolism*
  • Receptors, Transforming Growth Factor beta
  • Succinimides / metabolism
  • Transforming Growth Factors / metabolism
  • Transforming Growth Factors / pharmacology*
  • Tumor Cells, Cultured / cytology*
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / metabolism


  • Cross-Linking Reagents
  • Fibronectins
  • Receptors, Cell Surface
  • Receptors, Transforming Growth Factor beta
  • Succinimides
  • Transforming Growth Factors
  • disuccinimidyl suberate