Tetra primer ARMS-PCR relates folate/homocysteine pathway genes and ACE gene polymorphism with coronary artery disease

Mol Cell Biochem. 2011 Sep;355(1-2):289-97. doi: 10.1007/s11010-011-0866-6. Epub 2011 May 13.

Abstract

Cardiovascular disorders and coronary artery disease (CAD) are significant contributors to morbidity and mortality in heart patients. As genes of the folate/homocysteine pathway have been linked with the vascular disease, we investigated association of these gene polymorphisms with CAD/myocardial infarction (MI) using the novel approach of tetraprimer ARMS-PCR. A total of 230 participants (129 MI cases, 101 normal subjects) were recruited. We genotyped rs1801133 and rs1801131 SNPs in 5'10' methylenetetrahydrofolate reductase (MTHFR), rs1805087 SNP in 5' methyltetrahydrofolate homocysteine methyltransferase (MTR), rs662 SNP in paroxanse1 (PON1), and rs5742905 polymorphism in cystathionine beta synthase (CBS). Angiotensin converting enzyme (ACE) insertion/deletion polymorphism was detected through conventional PCR. Covariates included blood pressure, fasting blood sugar, serum cholesterol, and creatinine concentrations. Our results showed allele frequencies at rs1801133, rs1801131, rs1805087 and the ACE insertion/deletion (I/D) polymorphism varied between cases and controls. Logistic regression, after adjusting for covariates, demonstrated significant associations of rs1801133 and rs1805087 with CAD in the additive, dominant, and genotype model. In contrast, ACE I/D polymorphism was significantly related with CAD where recessive model was applied. Gene-gene interaction against the disease status revealed two polymorphism groups: rs1801133, rs662, and rs1805087; and rs1801131, rs662, and ACE I/D. Only the latter interaction maintained significance after adjusted for covariates. Our study concludes that folate pathway variants exert contributory influence on susceptibility to CAD. We further suggest that tetraprimer ARMS-PCR successfully resolves the genotypes in selected samples and might prove to be a superior technique compared to the conventional approach.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / genetics
  • Aryldialkylphosphatase / genetics
  • Case-Control Studies
  • Coronary Artery Disease / genetics*
  • Cystathionine beta-Synthase / genetics
  • Epistasis, Genetic
  • Female
  • Folic Acid / metabolism*
  • Gene Frequency
  • Genetic Association Studies
  • Genotype
  • Homocysteine / metabolism*
  • Humans
  • INDEL Mutation
  • Logistic Models
  • Male
  • Metabolic Networks and Pathways / genetics*
  • Methylenetetrahydrofolate Reductase (NADPH2) / genetics
  • Middle Aged
  • Peptidyl-Dipeptidase A / genetics*
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Single Nucleotide
  • Sequence Analysis, DNA / methods

Substances

  • Homocysteine
  • Folic Acid
  • Methylenetetrahydrofolate Reductase (NADPH2)
  • 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
  • Aryldialkylphosphatase
  • PON1 protein, human
  • Peptidyl-Dipeptidase A
  • Cystathionine beta-Synthase