Use of a Vero cell-based fluorescent assay to assess relative toxicities of Shiga toxin 2 subtypes from Escherichia coli

Beatriz Quiñones; Michelle S Swimley

doi:10.1007/978-1-61779-102-4_6

Use of a Vero cell-based fluorescent assay to assess relative toxicities of Shiga toxin 2 subtypes from Escherichia coli

Methods Mol Biol. 2011:739:61-71. doi: 10.1007/978-1-61779-102-4_6.

Authors

Beatriz Quiñones¹, Michelle S Swimley

Affiliation

¹ United States Department of Agriculture/Agricultural Research Service, Produce Safety and Microbiology Research Unit, Western Regional Research Center, Albany, CA, USA. Beatriz.Quinones@ars.usda.gov

PMID: 21567318
DOI: 10.1007/978-1-61779-102-4_6

Abstract

Shiga toxin-producing Escherichia coli is a leading cause of human gastroenteritis from food and waterborne sources worldwide. Shiga toxins 1 and 2 are important virulence factors linked to severe human illness. In particular, Shiga toxin 2 is composed of a diverse and heterogeneous group of subtypes with differential cytotoxicities in mammalian cells. In this chapter, we describe the use of the Vero-d2EGFP fluorescent assay to examine the relative toxicities of Stx2 and Stx2 subtypes expressed by strains of Shiga toxin-producing E. coli.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Chlorocebus aethiops
Food Contamination / analysis
Green Fluorescent Proteins / biosynthesis
Microscopy, Fluorescence / methods*
Shiga Toxin 1 / analysis
Shiga Toxin 1 / toxicity
Shiga Toxin 2 / analysis*
Shiga Toxin 2 / toxicity
Shiga-Toxigenic Escherichia coli / isolation & purification*
Shiga-Toxigenic Escherichia coli / pathogenicity*
Toxicity Tests
Vero Cells
Virulence Factors / analysis
Virulence Factors / toxicity

Substances

Shiga Toxin 1
Shiga Toxin 2
Virulence Factors
enhanced green fluorescent protein
Green Fluorescent Proteins