Anthocyanin-rich fractions of blackberry extracts reduce UV-induced free radicals and oxidative damage in keratinocytes

Phytother Res. 2012 Jan;26(1):106-12. doi: 10.1002/ptr.3510. Epub 2011 May 12.

Abstract

Hull blackberries were purified using solid phase extraction to obtain anthocyanin-rich methanol fractions. This method concentrated phenolics and anthocyanins, recovering 97% and 76% of the total yield in puree or powder extracts, respectively, which represented a 24-63 fold increase of the total antioxidant capacity when compared with either the water fraction or the original extract. The ability of these fractions to protect primary keratinocytes against UV-induced oxidative damage was assessed. Anthocyanin-rich methanol fractions derived from either blackberry powder or puree exhibited strong antioxidant properties, protecting against UV-induced ROS nearly as efficiently as N-acetyl cysteine. Furthermore, the fractions up-regulated the expression of catalase, MnSOD, Gpx1/2 and Gsta1 antioxidant enzymes. Thus, it is concluded that blackberry extracts may protect keratinocytes against UV-mediated oxidative damage.

MeSH terms

  • Acetylcysteine / pharmacology
  • Animals
  • Anthocyanins / isolation & purification
  • Anthocyanins / pharmacology*
  • Anthocyanins / therapeutic use
  • Antioxidants / metabolism*
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use
  • Catalase / metabolism
  • Fruit
  • Glutathione / metabolism
  • Keratinocytes / drug effects*
  • Keratinocytes / metabolism
  • Keratinocytes / radiation effects
  • Mice
  • Mice, Inbred C57BL
  • Oxidative Stress / drug effects*
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Radiation Injuries / drug therapy
  • Radiation Injuries / metabolism
  • Reactive Oxygen Species / metabolism
  • Rosaceae / chemistry*
  • Skin / drug effects
  • Skin / metabolism
  • Skin / radiation effects
  • Skin Diseases / etiology
  • Skin Diseases / metabolism
  • Skin Diseases / prevention & control*
  • Superoxide Dismutase / metabolism
  • Ultraviolet Rays
  • Up-Regulation

Substances

  • Anthocyanins
  • Antioxidants
  • Plant Extracts
  • Reactive Oxygen Species
  • Catalase
  • Superoxide Dismutase
  • Glutathione
  • Acetylcysteine