Abstract
Hull blackberries were purified using solid phase extraction to obtain anthocyanin-rich methanol fractions. This method concentrated phenolics and anthocyanins, recovering 97% and 76% of the total yield in puree or powder extracts, respectively, which represented a 24-63 fold increase of the total antioxidant capacity when compared with either the water fraction or the original extract. The ability of these fractions to protect primary keratinocytes against UV-induced oxidative damage was assessed. Anthocyanin-rich methanol fractions derived from either blackberry powder or puree exhibited strong antioxidant properties, protecting against UV-induced ROS nearly as efficiently as N-acetyl cysteine. Furthermore, the fractions up-regulated the expression of catalase, MnSOD, Gpx1/2 and Gsta1 antioxidant enzymes. Thus, it is concluded that blackberry extracts may protect keratinocytes against UV-mediated oxidative damage.
Copyright © 2011 John Wiley & Sons, Ltd.
MeSH terms
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Acetylcysteine / pharmacology
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Animals
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Anthocyanins / isolation & purification
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Anthocyanins / pharmacology*
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Anthocyanins / therapeutic use
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Antioxidants / metabolism*
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Antioxidants / pharmacology
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Antioxidants / therapeutic use
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Catalase / metabolism
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Fruit
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Glutathione / metabolism
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Keratinocytes / drug effects*
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Keratinocytes / metabolism
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Keratinocytes / radiation effects
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Mice
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Mice, Inbred C57BL
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Oxidative Stress / drug effects*
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
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Radiation Injuries / drug therapy
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Radiation Injuries / metabolism
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Reactive Oxygen Species / metabolism
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Rosaceae / chemistry*
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Skin / drug effects
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Skin / metabolism
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Skin / radiation effects
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Skin Diseases / etiology
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Skin Diseases / metabolism
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Skin Diseases / prevention & control*
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Superoxide Dismutase / metabolism
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Ultraviolet Rays
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Up-Regulation
Substances
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Anthocyanins
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Antioxidants
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Plant Extracts
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Reactive Oxygen Species
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Catalase
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Superoxide Dismutase
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Glutathione
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Acetylcysteine