PCR-RFLP to detect codon 248 mutation in exon 7 of p53 tumor suppressor gene

Liming Ouyang; Chongtao Ge; Haizhen Wu; Suxia Li; Huizhan Zhang

doi:10.1002/bmb.20255

PCR-RFLP to detect codon 248 mutation in exon 7 of p53 tumor suppressor gene

Biochem Mol Biol Educ. 2009 Mar;37(2):106-9. doi: 10.1002/bmb.20255.

Authors

Liming Ouyang¹, Chongtao Ge, Haizhen Wu, Suxia Li, Huizhan Zhang

Affiliation

¹ Department of Applied biology, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, People's Republic of China.

PMID: 21567714
DOI: 10.1002/bmb.20255

Abstract

Individual genome DNA was extracted fast from oral swab and followed up with PCR specific for codon 248 of p53 tumor suppressor gene. MspI restriction mapping showed the G-C mutation in codon 248, which closely relates to cancer susceptibility. Students learn the concepts, detection techniques, and research significance of point mutations or single nucleotide polymorphisms in individual genomes.