Protective effects of fucoxanthin against ferric nitrilotriacetate-induced oxidative stress in murine hepatic BNL CL.2 cells

Toxicol In Vitro. 2011 Oct;25(7):1314-9. doi: 10.1016/j.tiv.2011.04.023. Epub 2011 May 4.

Abstract

Fucoxanthin is a carotenoid that is rich in some seaweed. Although fucoxanthin has been reported to possess radical-scavenging activities in vitro, little is known whether it may protect against iron-induced oxidative stress in cultured cells. In this study, we examined the protection of fucoxanthin against oxidative damage in BNL CL.2 cells induced by ferric nitrilotriacetate (Fe-NTA). The data show that incubation of BNL CL.2 cells with Fe-NTA for 30 min significantly decreased cell proliferation, whereas pretreatment with fucoxanthin (1-20 μΜ) for 24h significantly recovered cell proliferation in a dose-dependent manner. In addition, fucoxanthin pretreatment significantly decreased intracellular reactive oxygen species (ROS) and DNA damage in BNL CL.2 cells incubated with Fe-NTA for 30 min. Moreover, fucoxanthin markedly decreased the level of thiobarbituric acid-reactive substances (TBARS) and protein carbonyl contents in BNL CL.2 cells induced by Fe-NTA. By contrast, fucoxanthin significantly increased the levels of GSH in a concentration-dependent manner. These results demonstrate that fucoxanthin at 1-20μΜ effectively prevents cytotoxicity in BNL CL.2 cells treated with Fe-NTA, and that the protective effect is likely associated with decreased intracellular ROS, TBARS, protein carbonyl contents and increased GSH levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Cell Line
  • Cell Proliferation / drug effects
  • Ferric Compounds / toxicity*
  • Glutathione / metabolism
  • Hepatocytes / drug effects*
  • Mice
  • Mutagens / toxicity*
  • Nitrilotriacetic Acid / analogs & derivatives*
  • Nitrilotriacetic Acid / toxicity
  • Oxidative Stress / drug effects*
  • Protein Carbonylation
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Xanthophylls / pharmacology*

Substances

  • Antioxidants
  • Ferric Compounds
  • Mutagens
  • Thiobarbituric Acid Reactive Substances
  • Xanthophylls
  • fucoxanthin
  • Glutathione
  • Nitrilotriacetic Acid
  • ferric nitrilotriacetate