Differential regulation of polysome mRNA levels in mouse Hepa-1C1C7 cells exposed to dioxin

Toxicol In Vitro. 2011 Oct;25(7):1457-67. doi: 10.1016/j.tiv.2011.04.020. Epub 2011 May 4.


The environmental agent 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD or dioxin) causes a multitude of human illnesses. In order to more fully understand the underlying biology of TCDD toxicity, we tested the hypothesis that new candidate genes could be identified using polysome RNA from TCDD-treated mouse Hepa-1c1c7 cells. We found that (i) differentially expressed whole cell and cytoplasm RNA levels are both poor predictors of polysome RNA levels; (ii) for a majority of RNAs, differential RNA levels are regulated independently in the nucleus, cytoplasm, and polysomes; (iii) for the remaining polysome RNAs, levels are regulated via several different mechanisms, including a "tagging" of mRNAs in the nucleus for immediate polysome entry; and (iv) most importantly, a gene list derived from differentially expressed polysome RNA generated new genes and cell pathways potentially related to TCDD biology.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Line
  • Cell Line, Tumor
  • Gene Expression Profiling
  • Gene Expression Regulation / drug effects
  • Hepatocytes / drug effects*
  • Mice
  • Oligonucleotide Array Sequence Analysis
  • Polychlorinated Dibenzodioxins / analogs & derivatives*
  • Polychlorinated Dibenzodioxins / toxicity
  • Polyribosomes / genetics
  • Polyribosomes / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*


  • Polychlorinated Dibenzodioxins
  • RNA, Messenger
  • 1,2,7,8-tetrachlorodibenzo-p-dioxin