Blautia stercoris sp. nov., isolated from human faeces

Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):776-779. doi: 10.1099/ijs.0.031625-0. Epub 2011 May 13.

Abstract

Strain GAM6-1T is a novel, strictly anaerobic, non-spore-forming, Gram-stain-positive bacterium that was isolated from the faeces of a healthy individual. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GAM6-1T was most closely related to Blautia producta ATCC 27340T (95.7 % sequence similarity), in the family Lachnospiraceae. Strain GAM6-1T did not exhibit catalase or oxidase activity. The strain's cellular fatty acids were of the straight-chain saturated and mono-unsaturated types, with C14:0 (24.10 %), C16:0 (19.09 %) and C16:0 dimethylacetal (14.35 %) predominant. Strain GAM6-1T was able to produce acid from various carbohydrates. Glucose fermentation produced acetic acid as the major short-chain fatty acid. The genomic DNA G+C content of strain GAM6-1T was 35.6 mol%. Based on phenotypic, genotypic and phylogenetic evidence, strain GAM6-1T (=KCTC 5981T=JCM 17204T) is considered to represent a novel species, for which the name Blautia stercoris sp. nov. is proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetic Acid / chemistry
  • Adult
  • Bacteria, Anaerobic / classification*
  • Bacteria, Anaerobic / genetics
  • Bacteria, Anaerobic / isolation & purification
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Feces / microbiology*
  • Fermentation
  • Genotype
  • Gram-Positive Bacteria / classification*
  • Gram-Positive Bacteria / genetics
  • Gram-Positive Bacteria / isolation & purification
  • Humans
  • Male
  • Molecular Sequence Data
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Acetic Acid

Associated data

  • GENBANK/HM626177