Chromosomal position effects are linked to sir2-mediated variation in transcriptional burst size

Biophys J. 2011 May 18;100(10):L56-8. doi: 10.1016/j.bpj.2011.04.021.

Abstract

Gene expression noise varies with genomic position and is a driving force in the evolution of chromosome organization. Nevertheless, position effects remain poorly characterized. Here, we present a systematic analysis of chromosomal position effects by characterizing single-cell gene expression from euchromatic positions spanning the length of a eukaryotic chromosome. We demonstrate that position affects gene expression by modulating the size of transcriptional bursts, rather than their frequency, and that the histone deacetylase Sir2 plays a role in this process across the chromosome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosomal Position Effects / genetics*
  • Chromosomes, Fungal / genetics*
  • Gene Expression Regulation, Fungal*
  • Models, Genetic
  • Saccharomyces cerevisiae / genetics*
  • Silent Information Regulator Proteins, Saccharomyces cerevisiae / genetics
  • Silent Information Regulator Proteins, Saccharomyces cerevisiae / metabolism*
  • Sirtuin 2 / genetics
  • Sirtuin 2 / metabolism*
  • Stochastic Processes
  • Transcription, Genetic*

Substances

  • Silent Information Regulator Proteins, Saccharomyces cerevisiae
  • SIR2 protein, S cerevisiae
  • Sirtuin 2