Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing

Fertil Steril. 2011 Jun 30;95(8):2564-7.e1-8. doi: 10.1016/j.fertnstert.2011.04.035. Epub 2011 May 14.

Abstract

We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blastocyst / physiology*
  • Cell Line
  • DNA Methylation*
  • Embryo Culture Techniques
  • Embryonic Stem Cells / physiology*
  • GRB10 Adaptor Protein / genetics
  • Gene Expression Regulation, Developmental*
  • Genomic Imprinting*
  • Humans
  • Potassium Channels, Voltage-Gated / genetics
  • Preimplantation Diagnosis / methods*
  • Proteins / genetics
  • RNA, Long Noncoding
  • Retinoblastoma Protein / genetics
  • Sequence Analysis, DNA / methods*
  • snRNP Core Proteins / genetics

Substances

  • GRB10 protein, human
  • KCNQ1OT1 long non-coding RNA, human
  • MEG3 non-coding RNA, human
  • Potassium Channels, Voltage-Gated
  • Proteins
  • RNA, Long Noncoding
  • Retinoblastoma Protein
  • SNRPN protein, human
  • snRNP Core Proteins
  • GRB10 Adaptor Protein