Complete Genome Sequence of the Marine Fish Pathogen Vibrio Anguillarum Harboring the pJM1 Virulence Plasmid and Genomic Comparison With Other Virulent Strains of V. Anguillarum and V. Ordalii

Infect Immun. 2011 Jul;79(7):2889-900. doi: 10.1128/IAI.05138-11. Epub 2011 May 16.

Abstract

We dissected the complete genome sequence of the O1 serotype strain Vibrio anguillarum 775(pJM1) and determined the draft genomic sequences of plasmidless strains of serotype O1 (strain 96F) and O2β (strain RV22) and V. ordalii. All strains harbor two chromosomes, but 775 also harbors the virulence plasmid pJM1, which carries the anguibactin-producing and cognate transport genes, one of the main virulence factors of V. anguillarum. Genomic analysis identified eight genomic islands in chromosome 1 of V. anguillarum 775(pJM1) and two in chromosome 2. Some of them carried potential virulence genes for the biosynthesis of O antigens, hemolysins, and exonucleases as well as others for sugar transport and metabolism. The majority of genes for essential cell functions and pathogenicity are located on chromosome 1. In contrast, chromosome 2 contains a larger fraction (59%) of hypothetical genes than does chromosome 1 (42%). Chromosome 2 also harbors a superintegron, as well as host "addiction" genes that are typically found on plasmids. Unique distinctive properties include homologues of type III secretion system genes in 96F, homologues of V. cholerae zot and ace toxin genes in RV22, and the biofilm formation syp genes in V. ordalii. Mobile genetic elements, some of them possibly originated in the pJM1 plasmid, were very abundant in 775, resulting in the silencing of specific genes, with only few insertions in the 96F and RV22 chromosomes.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Cholera Toxin / genetics
  • Chromosome Mapping
  • Chromosomes, Bacterial
  • DNA, Bacterial / genetics
  • Exonucleases / genetics
  • Fishes / microbiology
  • Genome, Bacterial*
  • Genomic Islands
  • Hemolysin Proteins / genetics
  • Molecular Sequence Data
  • O Antigens / genetics
  • Peptides / genetics
  • Plasmids*
  • Sequence Analysis, DNA
  • Vibrio / classification
  • Vibrio / genetics*
  • Vibrio / pathogenicity*
  • Virulence Factors / genetics

Substances

  • Ace toxin
  • Bacterial Proteins
  • DNA, Bacterial
  • Hemolysin Proteins
  • O Antigens
  • Peptides
  • Virulence Factors
  • zonula occludens toxin, Vibrio cholerae
  • anguibactin
  • Cholera Toxin
  • Exonucleases

Associated data

  • GENBANK/AEZA00000000
  • GENBANK/AEZB00000000
  • GENBANK/AEZC00000000
  • GENBANK/CP002284
  • GENBANK/CP002285