Generating mouse models of degenerative diseases using Cre/lox-mediated in vivo mosaic cell ablation

J Clin Invest. 2011 Jun;121(6):2462-9. doi: 10.1172/JCI45081. Epub 2011 May 16.

Abstract

Most degenerative diseases begin with a gradual loss of specific cell types before reaching a threshold for symptomatic onset. However, the endogenous regenerative capacities of different tissues are difficult to study, because of the limitations of models for early stages of cell loss. Therefore, we generated a transgenic mouse line (Mos-iCsp3) in which a lox-mismatched Cre/lox cassette can be activated to produce a drug-regulated dimerizable caspase-3. Tissue-restricted Cre expression yielded stochastic Casp3 expression, randomly ablating a subset of specific cell types in a defined domain. The limited and mosaic cell loss led to distinct responses in 3 different tissues targeted using respective Cre mice: reversible, impaired glucose tolerance with normoglycemia in pancreatic β cells; wound healing and irreversible hair loss in the skin; and permanent moderate deafness due to the loss of auditory hair cells in the inner ear. These mice will be important for assessing the repair capacities of tissues and the potential effectiveness of new regenerative therapies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alopecia / genetics
  • Alopecia / pathology
  • Animals
  • Apoptosis / genetics
  • Caspase 3 / deficiency
  • Caspase 3 / genetics*
  • Caspase 3 / physiology
  • Cell Lineage
  • Dimerization
  • Disease Models, Animal*
  • Epidermis / pathology
  • Gene Expression Regulation / drug effects
  • Gene Knockdown Techniques / methods*
  • Genes, Transgenic, Suicide*
  • Glucose Intolerance / genetics
  • Glucose Intolerance / pathology
  • Hair Cells, Auditory, Inner / pathology
  • Hearing Loss, Bilateral / genetics*
  • Hearing Loss, Sensorineural / genetics*
  • Homeodomain Proteins / genetics*
  • Insulin / deficiency
  • Insulin / genetics*
  • Islets of Langerhans / pathology
  • Keratin-14 / genetics*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic / genetics*
  • Mosaicism*
  • Organ Specificity
  • Phenotype
  • Tacrolimus / analogs & derivatives
  • Tacrolimus / pharmacology
  • Transcription Factor Brn-3C / deficiency
  • Transcription Factor Brn-3C / genetics*
  • Transgenes / drug effects
  • Wound Healing / genetics

Substances

  • AP20187
  • Homeodomain Proteins
  • Insulin
  • Keratin-14
  • Pou4f3 protein, mouse
  • Transcription Factor Brn-3C
  • Casp3 protein, mouse
  • Caspase 3
  • Tacrolimus