Completion of the primary structure of the human type IV collagenase preproenzyme and assignment of the gene (CLG4) to the q21 region of chromosome 16

Genomics. 1990 Mar;6(3):554-9. doi: 10.1016/0888-7543(90)90486-e.


The complete amino acid sequence of the human type IV collagenase preproenzyme was determined from cDNA and genomic clones. Primer extension and S1 nuclease analyses as well as nucleotide sequencing of a genomic clone indicate that the first exon has two closely spaced initiation sites for transcription and codes for 290 and 280 nt of a 5' untranslated region and a 29-residue signal peptide. The gene (CLG4) was localized to 16q21 using somatic cell hybrids and in situ hybridization.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 16*
  • Collagenases
  • DNA / genetics
  • Enzyme Precursors / genetics*
  • Genes
  • Humans
  • Hybrid Cells / analysis
  • Mice
  • Microbial Collagenase / genetics
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Protein Sorting Signals / genetics


  • Enzyme Precursors
  • Protein Sorting Signals
  • DNA
  • Collagenases
  • Microbial Collagenase

Associated data

  • GENBANK/M33789
  • GENBANK/M58552