The complete amino acid sequence of a post-synaptic neurotoxin isolated from the venom of the Australian death adder snake Acanthophis antarcticus

Comp Biochem Physiol B. 1990;95(1):45-50. doi: 10.1016/0305-0491(90)90246-p.

Abstract

1. A lethal neurotoxin (acanthophin d) was isolated from the venom of the Australian death adder snake Acanthophis antarcticus. 2. Acanthophin d consisted of a single polypeptide chain of 74 amino acid residues cross-linked by five disulphide bridges. 3. The results of neurophysiological experiments on murine phrenic nerve hemi-diaphragm preparations were consistent with irreversible post-synaptic blockage of neuromuscular transmission by acanthophin d.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Chemical Fractionation
  • Chymotrypsin
  • Elapid Venoms* / isolation & purification
  • Elapid Venoms* / pharmacology
  • In Vitro Techniques
  • Mice
  • Molecular Sequence Data
  • Neuromuscular Junction / drug effects
  • Neurotoxins* / isolation & purification
  • Peptides / analysis
  • Synaptic Transmission / drug effects
  • Trypsin

Substances

  • Amino Acids
  • Elapid Venoms
  • Neurotoxins
  • Peptides
  • acanthophin D
  • Chymotrypsin
  • Trypsin